首页 | 本学科首页   官方微博 | 高级检索  
     

基于混合抗体的酶联免疫分析方法同时检测孔雀石绿和隐孔雀石绿
引用本文:谢焕龙,王宇,徐振林,杨金易,肖治理,王弘,雷红涛,孙远明,沈玉栋.基于混合抗体的酶联免疫分析方法同时检测孔雀石绿和隐孔雀石绿[J].现代食品科技,2015,31(12):325-330.
作者姓名:谢焕龙  王宇  徐振林  杨金易  肖治理  王弘  雷红涛  孙远明  沈玉栋
作者单位:(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642),(华南农业大学食品学院,广东省食品质量安全重点实验室,广东广州 510642)
基金项目:国家自然科学基金(31371769);广东省科技计划项目(2012A020100002,2013B040402006);国家星火计划项目(2012GA780001);广州市科技计划项目(2013J4100053)
摘    要:孔雀石绿是"三致"物质,常被非法使用于渔业生产。水产品和环境中常以孔雀石绿和隐孔雀石绿两种形式同时存在,均具有强致癌性。但由于二者结构差异较大,现有的单克隆抗体只能分别检测样品中单一药物的残留量,难以客观地反映出孔雀石绿和隐孔雀石绿的总残留量。目前同时检测两种结构的免疫分析方法鲜有报道,本文分别制备了针对孔雀石绿和隐孔雀石绿的高特异性单克隆抗体,通过研究混合抗体模式建立了可以同时检测两种物质的酶联免疫分析方法,该方法对孔雀石绿和隐孔雀石绿混合物的半抑制浓度(50%inhibiting concentration,IC50)为3.27 ng/m L,检测线LOD(IC10)为0.24 ng/m L,线性范围为0.62~17.25 ng/m L。本方法的建立对保障水产品安全具有重要意义,也为混合抗体法同时检测两种或多种残留药物提供了方法借鉴。

关 键 词:孔雀石绿  隐孔雀石绿  单克隆抗体  混合抗体
收稿时间:2015/3/16 0:00:00

Simultaneous Detection of Malachite Green and Leucomalachite Green Based on Hybrid Antibody ELISA Analysis Method
XIE Huan-long,WANG Yu,XU Zhen-lin,YANG Jin-yi,XIAO Zhi-li,WANG Hong,LEI Hong-tao,SUN Yuan-ming and SHEN Yu-dong.Simultaneous Detection of Malachite Green and Leucomalachite Green Based on Hybrid Antibody ELISA Analysis Method[J].Modern Food Science & Technology,2015,31(12):325-330.
Authors:XIE Huan-long  WANG Yu  XU Zhen-lin  YANG Jin-yi  XIAO Zhi-li  WANG Hong  LEI Hong-tao  SUN Yuan-ming and SHEN Yu-dong
Affiliation:(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China),(College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China) and (College of Food Science, Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China)
Abstract:Malachite green (MG) is a carcinogenic, teratogenic, and mutagenic chemical that is often illegally used in fishery production. The chemical residues mainly exist in two forms, as MG and its metabolite leucomalachite green (LMG), both of which have severe carcinogenicity, in aquatic products and the environment. Due to the significant structural differences of these two compounds, existing monoclonal antibodies are only able to detect one drug residue in a sample, and cannot accurately reflect the total amount of MG and LMG residues. To date, immunoassay methods for simultaneous detection of MG and LMG residues have rarely been reported owing to the structural differences between the two. Highly specific monoclonal antibodies of MG and LMG were prepared separately, while the indirect competitive enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of MG and LMG was developed based on the hybrid antibody model. The half maximal inhibitory concentration (IC50) value of the established method on MG and LMG was 3.27 ng/mL, while the limit of detection (LOD) was 0.24 ng/mL and the linear range was 0.62~17.25 ng/mL. Thus, the establishment of the indirect competitive ELISA (icELISA) method is of great significance for ensuring the safety of aquatic food, in addition to providing a reference method to detect two or more residual chemicals simultaneously by the hybrid antibody assay.
Keywords:malachite green  leucomalachite green  monoclonal antibody  hybrid antibody
本文献已被 CNKI 等数据库收录!
点击此处可从《现代食品科技》浏览原始摘要信息
点击此处可从《现代食品科技》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号