Investigation of a tetracycline-regulated phage display system |
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| Authors: | Zahn, G. Skerra, A. Hohne, W. |
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| Affiliation: | 1 Institut für Biochemie (Charité), Humboldt-Universität zu Berlin, Monbijoustr.2, D-10117 Berlin and 2 Lehrstuhl für Biologische Chemie, Technische Universität München, An der Saatzucht 5, D-85350 Freising-Weihenstephan, Germany |
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| Abstract: | A new vector (pGZ1) was developed for bacterial phage displayof antibody fragments using a transcriptional regulation elementwith tight control. The tetp/°-based phasmid exhibits fullysuppressed scFv background synthesis in the absence of inducerand is independent of glucose as a catabolite repressor. Thevector is shown to be a useful alternative to commonly usedlacp/°-regulated systems. |
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| Keywords: | phagemid/ pHen/ single-chain Fv/ tetracycline promoter |
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