Start-up,adjustment and long-term performance of a two-stage bioremediation process,treating real acid mine drainage,coupled with biosynthesis of ZnS nanoparticles and ZnS/TiO2 nanocomposites |
| |
| Affiliation: | 1. Centro de Ciências do Mar, CCMAR, Portugal;2. Centro de Investigação Marinha e Ambiental, CIMA, Portugal;3. Centro de Investigação em Química do Algarve, CIQA, Portugal;4. Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;1. UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA Delft, The Netherlands;2. University of Cassino, Department of Mechanics, Structures and Environmental Engineering, via Di Biasio 43, 03043 Cassino (FR), Italy;1. Biological Process Laboratory, Department of Hydraulic and Sanitation, Sao Carlos School of Engineering (EESC), University of Sao Paulo (USP), Brazil;2. Departament of Sea Science, Federal University of São Paulo, Av. Saldanha da Gama, 89, Ponta da Praia 11030-400, Santos, SP, Brazil;1. Department of Geology, Southern Illinois University, Carbondale, IL 62901, USA;2. Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA;3. Environmental Resources and Policy Program, Southern Illinois University, Carbondale, IL 62901, USA |
| |
| Abstract: | Acid mine drainage (AMD) generation is a widespread environmental problem in Europe, including Portugal. Previous experience has shown that a combined process consisting of an anaerobic sulphate-reducing bioreactor, following neutralization with calcite tailing, produces water complying with legal irrigation requirements from synthetic AMD. Aiming the treatment of real AMD a new bioreactor was inoculated with a SRB enrichment obtained from sludge from a local WWTP anaerobic lagoon. In the initial batch phase, sulphate supplementation was needed to achieve high sulphate-reducing bacteria counts before continuous feeding of AMD was started. The system quickly achieved good performance, proving it is easy to start-up. However, this time the neutralization step failed to keep bioreactor affluent pH higher than 5 for longer than three weeks. This was due to armouring of calcite by precipitates of various metals present in AMD. A new configuration, replacing a packed-bed column by a shallow contact basin, proved to be more robust, avoiding clogging, short-circuiting and providing long-term neutralization. The treated effluent, with excess of biologically generated sulphide, was successfully used to synthesize zinc sulphide nanoparticles, both in pure form and as a ZnS/TiO2 nanocomposite, thus proving the feasibility of coupling an AMD bioremediation system with the synthesis of metal sulphide nanoparticles and nanocomposites. |
| |
| Keywords: | Bioremediation Acid mine drainage Sulphate-reducing bacteria ZnS nanoparticles |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
