Parameter optimization for the enzymatic hydrolysis of sunflower oil in high-pressure reactors |
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Authors: | Mateja?Primo?i? Maja?Habulin Email author" target="_blank">?eljko?KnezEmail author |
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Affiliation: | (1) Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia |
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Abstract: | This study is concerned with the hydrolysis of sunflower oil in the presence of lipase preparation Lipolase 100T (Aspergillus niger lipase). Supercritical carbon dioxide was used as a solvent for this reaction. In a high-pressure stirred tank reactor operated
in a batch mode, the effects of various process parameters (temperature, pressure, enzyme/substrate ratio, pH, and oil/buffer
ratio) were investigated to determine the optimal reaction rate and conversion for the hydrolysis process. The optimal concentration
of lipase was 0.0714 g/mL of CO2-free reaction mixture, and the highest conversions of oleic acid (0.193 g/g of oil phase) and linoleic acid (0.586 g/g of
oil phase) were obtained at 50°C, 200 bar, pH=7, and an oil/buffer ratio of 1∶1 (w/w). |
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Keywords: | High-pressure hydrolysis lipase sunflower oil supercritical carbon dioxide |
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