石榴籽油的体内抗氧化性评价

利用水酶法提取石榴籽油,以5、10、15 mL/(kg.bw.d)3种剂量的石榴籽油分别给低剂量组(5 mL/(kg.bw.d))、中剂量组(10 mL/(kg.bw.d))和高剂量组(15 mL/(kg.bw.d))的昆明小鼠经口灌胃,空白对照组以蒸馏水灌胃,30 d后全部小鼠颈椎脱臼处死,快速取出肝、脑组织并测定其中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性和丙二醛(MDA)的含量。结果显示:低剂量石榴籽油试验组小鼠肝、脑组织中SOD、GSH-PX等抗氧化酶活性明显高于正常对照组(p0.05),而脑中的MDA含量明显低于正常对照组(p0.05)。表明适当剂量石榴籽油可以增强机体的抗氧化机能,从而延缓衰老的发生。

Assessment on antioxidant activity of pomegranate seed oil in vivo

Pomegranate seed oil extracted through aqueous enzymatic method was administered to the low-dose group.middle-dose group and high-dose group of Kunming mouse by oral gavage every day at three consecutive doses of 5,10,15 mL/(kg·bw·d),while the control group was given distilled wa-ter.After 30 days,all mices were killed through cervical dislocation,and liver and brain tissues were quickly removed and assayed for their superoxide dismutase(SOD),glutathione peroxidase(GSH-PX) activities and malondialdehyde(MDA)contents.The results showed that SOD,GSH-PX activities in liv-er and brain tissues of the low-dose group were significantly higher than that of the control group(P<0.05),and MDA contents in brain tissues were significantly lower than that of the control group(P<0.05).It indicated that an appropriate dose of pomegranate seed oil could enhance the antioxidant func-tion of the body,thus had an anti-aging ability.