Fate of Tannins in Corsican Pine Litter |
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Authors: | Klaas G. J. Nierop Jacobus M. Verstraten |
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Affiliation: | (1) Institute for Biodiversity and Ecosystem Dynamics (IBED)-Earth Surface Processes and Materials (ESPM), Universiteit van Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands |
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Abstract: | Tannins are ubiquitous in higher plants and also in litter and soils where they affect many biogeochemical processes. Despite this well-recognized role, their fate in litter and mineral soils is hardly known, as often only trace amounts, if any, are measured. In this study, we conducted an incubation experiment with Corsican pine litter to which known amounts of tannic acid (TA) or condensed tannins (CTs) from Corsican pine were added. Using Folin–Ciocalteu as a measure for total phenolics and HCl–butanol as an assay specific for CTs, acetone/water extractable phenolics and tannins decreased with time towards very low levels. Application of thermally assisted hydrolysis and methylation to litter before and after acetone/water extraction revealed that TA concentration decreased. By contrast, CTs remained to a great extent in the litter and could not be extracted suggesting that they were tightly bound. |
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Keywords: | Condensed tannins Tannic acid Thermally assisted hydrolysis and methylation Corsican pine litter |
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