Identification of a Female-Specific, Antennally Active Volatile Compound of the Currant Stem Girdler |
| |
Authors: | Allard A Cossé Robert J Bartelt David G James Richard J Petroski |
| |
Affiliation: | (1) National Center for Agricultural Utilization Research, Bioactive Agents Research Unit, USDA Agricultural Research Service, 1815 N. University Street, Peoria, Illinois, 61604;(2) Irrigated Agriculture Research and Extension Center, Washington State University, 24106 North Bunn Road, Prosser, Washington, 99350 |
| |
Abstract: | We identified (Z)-9-octadecen-4-olide as a female-specific, antennally active compound from the currant stem girdler Janus integer Norton. Female specificity was demonstrated by gas chromatographic comparison of liquid chromatography fractions of male and female volatile emissions and whole body extracts. The -lactone was identified by coupled gas chromatographic-electroantennographic detection (GC-EAD), coupled gas chromatographic-mass spectrometric (GC-MS) analysis, microchemical reactions, and GC and MS comparison with authentic standards. GC-EAD analysis of female volatile emissions and cuticular extracts showed a single peak of activity on male antennae, which was not present in male-derived materials. Female antennae did not respond to any of the tested materials. The hydrogenation product of the natural EAD-active material was a known saturated -lactone. The mass spectrum of the dimethyl disulfide derivative of the natural -lactone was consistent with a double bond present in the 9 position. Comparison of the natural -lactone and a synthesized racemic mixture of (Z)-9-octadecen-4-olide on a chiral GC column showed the presence of a single enantiomer in the natural material. |
| |
Keywords: | Janus integer sawfly Hymenoptera Cephidae sex pheromone (Z)-9-octadecen-4-olide coupled gas chromatographic-electroantennographic detection (GC-EAD) |
本文献已被 SpringerLink 等数据库收录! |
|