| 玉米醇溶蛋白湿热法糖基化初步研究 |
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| 引用本文： | 孔繁惠,刘景圣,修琳,闵伟红. 玉米醇溶蛋白湿热法糖基化初步研究[J]. 中国食物与营养, 2012, 18(4): 55-58 |
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| 作者姓名： | 孔繁惠 刘景圣 修琳 闵伟红 |
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| 作者单位： | 吉林农业大学食品科学与工程学院,长春150118／小麦玉米深加工国家工程实验室,长春130118 |
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| 基金项目： | 国家高新技术研究发展计划“玉米绿色供应链技术创新与装备研制”(项目编号:2008AA100802) |
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| 摘 要： | 对玉米醇溶蛋白湿法糖基化的改性工艺进行了初步研究,分析了不同糖基供体、温度、底物浓度对接枝度、可溶性蛋白和游离氨基含量的影响。结果表明,葡萄糖作为糖基供体,在90℃、蛋白与葡萄糖质量比为1：2时进行糖基化反应,接枝度可达到38.34%,可溶性蛋白含量为1.22mg/mL,可溶性蛋白含量提高了1.84倍。
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| 关 键 词： | 玉米醇溶蛋白 糖基化 接枝度 |
Preliminary Research on Glycosylation of Zein by Water-heating |
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| KONG Fan-hui,LIU Jing-sheng,XIU Lin,MIN Wei-hong. Preliminary Research on Glycosylation of Zein by Water-heating[J]. Food and Nutrition in China, 2012, 18(4): 55-58 |
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| Authors: | KONG Fan-hui LIU Jing-sheng XIU Lin MIN Wei-hong |
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| Affiliation: | 1,2 (1 College of Food Science and Engineering,Jilin Agricultural University,Jilin 130118,China; 2 National Engineering Laboratory on Wheat and Corn Further Processing,Jilin 130118,China) |
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| Abstract: | Modification process of glycosylation of zein by water-heating was preliminarily studied.Effects of different glycosyl donor,temperature,concentration of substrate on the conjugation degrees and the solubility and free amino of the conjugates were investigated.Results showed that under these conditions(temperature 90℃,weight ratio of protein to glucose 1:2) with glucose as glycosyl donor,conjugation degrees achieved 38.34% and content of soluble protein was 1.22mg/mL which rised 1.84 times than original zein. |
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| Keywords: | zein glycosylation conjugation degrees |
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