| 天麻糖蛋白GGE2b的分离纯化及对急性血瘀大鼠血液流变的影响 |
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| 引用本文： | 丁诚实.天麻糖蛋白GGE2b的分离纯化及对急性血瘀大鼠血液流变的影响[J].食品科学,2010,31(1):240-242. |
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| 作者姓名： | 丁诚实 |
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| 作者单位： | 枣庄学院生命科学系 |
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| 摘 要： | 目的：从天麻中分离纯化得到糖蛋白组分GGE2b，研究其对急性血瘀大鼠血液流变学指标的影响。方法：采用离子交换和快速蛋白液相层析色谱(FPLC)技术分离天麻水溶性提取物，得到组分GGE2b。采用皮下注射盐酸肾上腺素结合冷刺激的方法制造急性血瘀大鼠模型。观察GGE2b 各剂量组对急性血瘀大鼠全血黏度、血浆黏度、红细胞压积、红细胞聚集指数和红细胞变形指数的影响。结果：离子交换结合FPLC 技术分离纯化得到天麻糖蛋白组分GGE2b。GGE2b 60、120mg/kg 能够显著降低急性血瘀模型大鼠高切、中切的全血黏度(P ＜ 0.05 或0.01)和血浆黏度(P ＜ 0.01)，降低红细胞聚集指数(P ＜ 0.05 或0.01)，增大红细胞变形指数(P ＜ 0.05)。结论：层析技术用于天麻糖复和物的分离纯化是可行的，且得到的糖蛋白组分GGE2b对急性血瘀大鼠异常的血液流变有良好的治疗效果。
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| 关 键 词： | 天麻糖蛋白 快速蛋白液相色谱 急性血瘀 血液流变 |
| 收稿时间： | 2009-02-18 |
Isolation,Purification and Hemorrheological Effect of Glycoprotein from Gastrodia elata (GGE2b) in Blood Stasis Rats |
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| DING Cheng-shi.Isolation,Purification and Hemorrheological Effect of Glycoprotein from Gastrodia elata (GGE2b) in Blood Stasis Rats[J].Food Science,2010,31(1):240-242. |
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| Authors: | DING Cheng-shi |
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| Affiliation: | (Department of Life Science, Zaozhuang University, Zaozhuang 277160, China) |
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| Abstract: | Objective:To isolate and purify glycoprotein from Gastrodia elata(GGE2b) and investigate its hemorrheological effect in acute blood stasis rats.Methods:Soluble extracts of Gastrodia elata were purified by DEAE-Sepharose column chromatography and fast protein liquid chromatography(FPLC) to obtain GGE2b.Acute blood stasis rat model was developed by hypodermic adrenaline injection combined with cold-shock treatment.Totally 60 male rats were randomly divided into 6 groups of 10 rats each,namely saline group,acu... |
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| Keywords: | glycoprotein from Gastrodia elata fast protein liquid chromatography acute blood stasis hemorrheology |
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