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Application of design‐based stereology for estimation of absolute volume and surface area of the articular and calcified cartilage compartments of undecalcified human femoral heads
Authors:L.B. HARTLEV  J.R. NYENGAARD  J.S. THOMSEN  L.W.T. BOEL  K. STENGAARD‐PEDERSEN  E.‐M. HAUGE
Affiliation:1. Department of Rheumatology, Aarhus University Hospital, , Aarhus, Denmark;2. Stereology and Electron Microscopy Laboratory, Center for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, , Aarhus, Denmark;3. Department of Biomedicine – Anatomy, Aarhus University, , Aarhus, Denmark;4. Institute of Forensic Medicine, Aarhus University, , Aarhus, Denmark
Abstract:Design‐based stereological methods using systematic uniform random sampling, the Cavalieri estimator and vertical sections are used to investigate undecalcified human femoral heads. Ten entire human femoral heads, obtained from normal women and normal men, were systematically sampled and thin undecalcified vertical sections were obtained. Absolute volumes and surface areas of the entire femoral head, the articular cartilage and the calcified cartilage compartments were estimated. In addition, the average thickness of the articular cartilage and the calcified cartilage were calculated. The stereological procedures applied to the human femoral heads resulted in average coefficient of errors, which were 0.03–0.06 for the volume estimates and 0.03–0.04 for the surface area estimates. We conclude that design‐based stereology using the Cavalieri estimator and vertical sections can successfully be used in large undecalcified tissue specimens, like the human femoral head, to estimate the absolute volume and surface area of macroscopic as well as of microscopic tissue compartments. The application of well‐known design‐based stereological methods carries potential advantage for investigating the pathology in inflammatory and degenerative joint diseases.
Keywords:Articular cartilage  calcified cartilage  Cavalieri estimator  femoral head  quantitative microscopy  systematic uniform random  vertical sections
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