Analysis of lipids containing hydroxy fatty acids |
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| Authors: | Fasih Ahmad René Maier Kumar D Mukherjee Helmut K Mangold |
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| Affiliation: | (1) Bundesanstalf fuer Fettforschung, Institut fuer Biochemie und Technologie, H.P. Kaufmann-Institut, Piusallee 68, D-4400 Muenster, Federal Republic of Germany;(2) The Hormel Institute, University of Minnesota, Austin, Minnesota;(3) Present address: Section of Oils and Fats, Department of Chemistry, Aligarh Muslim University, 20200 Aligarh, India;(4) Present address: Ciba-Geigy, A.G., CH-4000 Basel, Switzerland;(5) Present address: Bundesanstalt fuer Fettforschung, D-4400 Muenster, Federal Republic of Germany |
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| Abstract: | Lipids containing hydroxy fatty acids or hydroxyacyl moieties are acetylated with 1-14C]acetic anhydride or 3H]acetic anhydride, and the content of hydroxy fatty acids or hydroxyacyl moieties is estimated from the specific radioactivity
of the acetylated products with respect to that of radioactive standards, such as radioacetylated ricinoleic acid or triricinoleoylglycerol.
Mixtures of triacylglycerols containing one, two and three hydroxyl groups per molecule are derivatized in a similar manner,
and the resulting acetates are fractionated by thin layer chromatography according to the number of acetate groups per molecule.
The relative proportion of each type of triacylglycerols in the mixture is estimated from the distribution of radioactivity
in the various fractions. Applications of these techniques are demonstrated by the analysis of several seed lipids. |
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