Evaluation of spectral imaging for plant cell analysis |
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| Authors: | R. H. Berg |
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| Affiliation: | Integrated Microscopy Facility, Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, U.S.A. |
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| Abstract: | Fluorescence imaging at high spectral resolution is now a practical reality and has great promise in plant cell biology. Emission spectral curve data can be used computationally to distinguish spectrally similar fluorophores, or to remove autofluorescence, and to spectrally analyse autofluorescent molecules, which are especially abundant in plant tissues. Examples of these applications in plant cells are given, and a comparison is made between the current offerings in spectral imaging laser scanning confocal microscopes. |
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| Keywords: | Autofluorescence confocal microscopy multiphoton microscopy spectral imaging unmixing |
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