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HPLC法测定梅鹿辄和黑比诺葡萄中的花色素苷
引用本文:王贞强,韩富亮,赵仁邦,桑亚新,刘卫华.HPLC法测定梅鹿辄和黑比诺葡萄中的花色素苷[J].酿酒科技,2007(5):99-101.
作者姓名:王贞强  韩富亮  赵仁邦  桑亚新  刘卫华
作者单位:1. 河北农业大学食品科技学院,河北,保定,071001
2. 中国农业大学食品科学与营养工程学院,北京,100086
摘    要:利用甲醇和超声波提取酿酒葡萄梅鹿辄和黑比诺中的花色素苷,利用高效液相色谱测定该葡萄中的花色素苷.在梅鹿辄葡萄果实中共检测到9种花色素苷,以二甲花翠素3-O-葡萄糖苷、二甲花翠素3-O-(6-O-乙酰)葡萄糖苷和二甲花翠素3-O-(6-O-对香豆酰)葡萄糖苷为主,这三者占总成分的79.69%,构成了梅鹿辄葡萄果实的主要呈色成分.在黑比诺果实中只检测到5种花色素苷,其中二甲花翠素3-O-葡萄糖苷和甲基花青素3-O-葡萄糖苷构成了黑比诺葡萄果 实的主要呈色物质,占总成分的94.18%.

关 键 词:酿酒葡萄  葡萄酒  梅鹿辄  黑比诺  花色素苷  HPLC  色谱测定  梅鹿辄  黑比诺  葡萄糖苷  花色素苷  Grape  Anthocyanin  呈色物质  花青素  甲基  构成  成分  香豆酰  乙酰  检测  葡萄果实  高效液相  酿酒葡萄  提取
文章编号:1001-9286(2007)05-0099-03
修稿时间:2007-01-08

Determination of Anthocyanin in Merlot Grape and in Pinot Noir Grape by HPLC
WANG Zhen-qiang,HAN Fu-liang,ZHAO Ren-bang,SANG Ya-xin,LIU Wei-hua.Determination of Anthocyanin in Merlot Grape and in Pinot Noir Grape by HPLC[J].Liquor-making Science & Technology,2007(5):99-101.
Authors:WANG Zhen-qiang  HAN Fu-liang  ZHAO Ren-bang  SANG Ya-xin  LIU Wei-hua
Abstract:Anthocyanin in Merlot grape and in Pinot Noir grape was extracted by methanol and ultrasonic and then it was detected by HPLC.9 kinds of anthocyanins were detected in Merlot grape mainly including Malvidin 3-O-Glu,Malvidin 3-O-(6-O-Acetyl)-Glu and Malvidin 3-O-(6-O-P-Coumaryl)-Glu which accounted for 76.69 % of the total anthocyanins and were the main coloration compositions of Merlot grape.Only 5 kinds of anthocyanins were detected in Pinot Noir grape mainly including Malvidin 3-O-Glu and Peonidin 3-O-Glu(the main coloration compositions) which accounted for 94.18 % of the total anthocyanins.
Keywords:wine-making grape  grape wine  Merlot  Pinot nior  antlaocyanin
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