| 洋河酒厂成品大曲水解酶系研究 |
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| 引用本文： | 周新虎,陈翔,赵星辉,葛向阳.洋河酒厂成品大曲水解酶系研究[J].酿酒,2011,38(5):23-26. |
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| 作者姓名： | 周新虎 陈翔 赵星辉 葛向阳 |
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| 作者单位： | 江苏洋河酒厂股份有限公司,江苏宿迁,223800 |
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| 摘 要： | 采用盐析、离子交换、分子筛筛选等方法分离、纯化洋河大曲中糖化淀粉酶、液化淀粉酶和酸性蛋白酶,并研究了3种酶的相关酶学特性。研究结果表明,成品洋河大曲中,糖化淀粉酶活力均值为441.0u/g干曲,液化淀粉酶活力为5.68u/g干曲,酸性蛋白酶活力均值达51.2u/g干曲。糖化淀粉酶纯化倍数平均达2.44倍,酶活回收率2.51%,表观分子量2.5×104,液化淀粉酶的纯化倍数平均达3.24倍,酶活回收率28.28%。酸性蛋白酶纯化倍数平均达4.05倍,酶活回收率可达35.62%。
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| 关 键 词： | 浓香型白酒 葡糖淀粉酶 α-淀粉酶 酸性蛋白酶 |
Luzhou-flavor Daqu in the Separation and Purification of Three Kinds of Enzymes |
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| ZHOU Xin-hu,CHENG Xiang,ZHAO Xing-hui,GE Xiang-yang.Luzhou-flavor Daqu in the Separation and Purification of Three Kinds of Enzymes[J].Liquor Making,2011,38(5):23-26. |
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| Authors: | ZHOU Xin-hu CHENG Xiang ZHAO Xing-hui GE Xiang-yang |
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| Affiliation: | ZHOU Xin-hu1,CHENG Xiang1,ZHAO Xing-hui1,GE Xiang-yang1,2(1.Yanghe Distillery Co.Ltd.,Suqian,Jiangsu 223800,China,2.Jiangnan University,Jiangsu,Wuxi 214122,China) |
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| Abstract: | elaborated the salting-out,ion-exchange,molecular sieve screening methods such as separation,purification of Luzhou-flavor Daqu of amylase,liquefying amylase and acidic protease,and 3 kinds of enzyme enzymatic characteristics.The results show that,the finished product flavor Daqu in saccharifying amylase activity,mean 441u(g / stem yeast),liquefied amylase activity was 5.68 u(g / stem yeast),acid protease activity mean of 51.2u(g / stem yeast).Saccharifying amylase purification multiples is amounted to on a... |
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| Keywords: | Luzhou-flavor liquor Daqu glucoamylase α-amylasea cid protease |
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