| 根霉ZZ-3脂肪酶发酵条件的优化及酶学性质研究 |
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| 引用本文： | 魏涛,封盛雪,毛多斌,魏东芝.根霉ZZ-3脂肪酶发酵条件的优化及酶学性质研究[J].食品工程,2011(2):20-23. |
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| 作者姓名： | 魏涛 封盛雪 毛多斌 魏东芝 |
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| 作者单位： | 郑州轻工业学院食品与生物工程学院,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目资助(102102210063); 郑州轻工业学院博士科研基金(000544) |
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| 摘 要： | 对根霉ZZ-3脂肪酶产酶条件的优化及脂肪酶酶学性质进行了研究。结果表明,250 mL三角瓶液体培养,培养基装液量100 mL,根霉ZZ-3产脂肪酶的最佳培养基为：黄豆粉3 g/100mL,（NH4）2S040.3 g/100mL,MgS04 0.2 g/100mL,K2HPO4 0.2 g/100mL,花生油0.3 g/100mL,pH自然。在温度28℃、转速150 r/min,摇床培养时间2 d,发酵酶活力达到124.60 U/mL。对该酶的酶学性质进行了初步研究,该酶最适pH7.5,最适反应温度37℃,在pH7～9、温度低于40℃条件下酶活稳定。
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| 关 键 词： | 根霉 脂肪酶 产酶条件 酶学性质 |
Production conditions and enzymatic properties of lipase from rhizopus ZZ-3 |
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| WEI Tao,FENG Sheng-xue,MAO Duo-bin,WEI Dong-zhi.Production conditions and enzymatic properties of lipase from rhizopus ZZ-3[J].Food Engineering,2011(2):20-23. |
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| Authors: | WEI Tao FENG Sheng-xue MAO Duo-bin WEI Dong-zhi |
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| Affiliation: | WEI Tao FENG Sheng-xue MAO Duo-bin WEI Dong-zhi(College of food science and engineering,Shanxi agricultural university,Taigu 030801,China) |
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| Abstract: | In this paper the production conditions of lipase from Rhizopus ZZ-3 were optimized and its enzymatic properties were studied.The results indicated that the suitable conditions for lipase production from Rhizopus were as the follows： soy powder 3 g /100mL,（NH4）2S04 0.3 g /100mL,MgS04 0.2 g /100mL,K2HPO4 0.2 g /100mL,soybean 0.3 g /100mL,pH natural.The highest lipase activity was 124.60 U /mL on the condition of 28 ℃,150 r / min.The properties of the lipase was also characterized.The optimal temperature and pH of lipase was 37 ℃ and 7.5.The lipase was relatively stable in pH range of 7-9 and at 40 ℃. |
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| Keywords: | rhizopus lipase productions of lipase enzymatic properties |
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