Fluorescence study of interaction between an anionic conjugated polyelectrolyte and bovine serum albumin |
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| Authors: | Yani Wang Jie Dong Chuanzhen Liu Biqing Bao Lianhui Wang Xiaowei Zhan Huai Yang Guojie Wang |
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| Affiliation: | (1) School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China;(2) Beijing BOE Display Technology Co., Ltd, Beijing, 100176, China;(3) Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, China;(4) CAS Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; |
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| Abstract: | The interaction between an anionic conjugated polyelectrolyte, poly9,9-bis(3′-butyrate)fluoren-2,7-yl] sodium (BBS-PF), and
bovine serum albumin was investigated by fluorescence spectroscopy. The emission of BBS-PF was effectively quenched by BSA
with a quenching constant K
SV of 3.1 × 107 L/mol when BSA was at nanomolar concentrations, but the emission increased when the concentration of BSA was at micromolar
level. The excitation band of BBS-PF blue-shifted when the emission was quenched where the negatively charged BSA induced
the aggregation of BBS-PF, yet the excitation band of BBS-PF red-shifted when the emission increased where the BSA acted as
a surfactant and formed hydrophobic interaction with BBS-PF. BBS-PF could also quench BSA through energy transfer by resonance
with a quenching constant K
SV of 1.1 × 106 L/mol. The emission band changes of BSA reflected the conformation transitions of BSA from class II to class I and the binding
of BBS-PF with BSA made the BSA more folded. |
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