蜂蜜中17种水解氨基酸的测定

目的采用酸水解法和全自动氨基酸分析仪同时测定蜂蜜中17种氨基酸的含量。方法 8种不同蜜源的蜂蜜经酸水解后,采用全自动氨基酸分析仪进行定量检测;检测器为荧光检测器,脯氨酸在波长440 nm处测定,其余16种氨基酸在570 nm波长下测定。结果 17种氨基酸的线性回归系数均达到0.99以上;方法检出限为5~10 nmoL/L,样品平均回收率为92.1%~99.3%,相对标准偏差RSD小于8.5%。经检测实际样品发现,蜂蜜中不含胱氨酸,氨基酸总量在32.214~78.439 mg/100 g范围。结论该方法快速、准确,可适用于蜂蜜中水解氨基酸含量的测定。

Determination of 17 kinds of hydrolytic amino acids in honey

Objective To establish a method for simultaneous determination of 17 kinds of hydrolytic amino acids by acid hydrolyzation and amino acid analyzer. Methods Eight different sources of honey samples were hydrolyzed with acid, and the quantitative detection was carried out with a full automatic amino acid analyzer. Proline was detected by fluorescence detector at the wavelength of 440 nm while the other 16 kinds of amino acid were detected at the wavelength of 570 nm. Results Linear regression coefficients of 17 kinds of hydrolytic amino acids were all above 0.99, the limits of detection were 5~10 nmoL/L, and the recovery rates of samples were 92.1%~99.3% with RSDs less than 8.5%. By detecting the actual samples, it was found that total amino acid of honey was in the range of 32.214~78.439 mg/100 g excluding cystine. Conclusion The method is rapid and accurate, which can be used for the determination of hydrolytic amino acids in honey.