| 宁海白枇杷花中酚类物质鉴定及含量测定 |
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| 引用本文： | 闫永芳,过尘杰,孙钧,孟天真,赵春江,叶兴乾. 宁海白枇杷花中酚类物质鉴定及含量测定[J]. 中国食品学报, 2012, 12(4): 202-207 |
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| 作者姓名： | 闫永芳 过尘杰 孙钧 孟天真 赵春江 叶兴乾 |
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| 作者单位： | 浙江大学生物系统工程与食品科学学院 杭州 310058 |
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| 基金项目： | 教育部创新工程重大项目培育资金，浙江省重大科技专项 |
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| 摘 要： | 运用HPLC-PAD-MS技术对宁海白枇杷花中酚类化合物进行定性及定量分析,共检测到6种主要酚类化合物,4种被鉴定。其中,异槲皮苷含量最高,达到1.536mg/g;绿原酸0.5550mg/g;槲皮苷0.5836mg/g;槲皮素含量较低,0.2294mg/g。通过回收率试验、系统稳定性试验及日间精密度试验对该方法进行验证,结果显示各物质的回收率在90.3%～101.2%之间,方法准确性好;各标品峰高及峰面积的变异系数均小于1%(n=3),系统稳定性较好;各标品保留时间的变异系数均小于1%,日间精密度良好。本分析方法具有快速、灵敏、准确的优点,适用于枇杷花中酚类物质的定性及定量分析。
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| 关 键 词： | 酚类物质 枇杷花 HPLC-PAD-MS |
Identification and Determination of Phenols in Loquat Flower by HPLC-PAD-MS |
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| Yan Yongfang , Guo Chenjie , Sun Jun , Meng Tianzhen , Zhao Chunjiang , Ye Xingqian. Identification and Determination of Phenols in Loquat Flower by HPLC-PAD-MS[J]. Journal of Chinese Institute of Food Science and Technology, 2012, 12(4): 202-207 |
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| Authors: | Yan Yongfang Guo Chenjie Sun Jun Meng Tianzhen Zhao Chunjiang Ye Xingqian |
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| Affiliation: | Yan Yongfang Guo Chenjie Sun Jun Meng Tianzhen Zhao Chunjiang Ye Xingqian*(College of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310058) |
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| Abstract: | Phenols in NingHai white loquat flower were identified and determined using a HPLC-PAD-MS method in the research.Six compounds were detected and four of them were identified.The identified phenols were chlorogenic acid(0.5550 mg/g),quercetin-3-o-glucoside(1.536 mg/g),quercitrin(0.5836 mg/g) and quercetin(0.2294 mg/g).The recovery test was between 90.3% and 101.2%,and the CV of stability and inter-day repeatability were lower than 1%.The results showed that the established method was a fast,effective and accurate method to analyze phenols in loquat flower. |
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| Keywords: | phenols loquat flower HPLC-PAD-MS |
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