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| 1种检测橄榄油掺假的新方法——PCR-CE-SSCP法 | |
| 引用本文： | 张海亮,吴亚君,鞠兴荣,王斌,韩建勋,陈颖.1种检测橄榄油掺假的新方法——PCR-CE-SSCP法[J].中国食品学报,2012,12(3):151-159. |
| 作者姓名： | 张海亮 吴亚君 鞠兴荣 王斌 韩建勋 陈颖 |
| 作者单位： | 1. 南京财经大学食品科学与工程学院 南京210003 ;中国检验检疫科学研究院 北京100123 2. 中国检验检疫科学研究院 北京100123 3. 南京财经大学食品科学与工程学院 南京210003 |
| 基金项目： | 科技部质检公益项目，863计划项目 |
| 摘 要： | 橄榄油真伪鉴别问题一直是食品真伪鉴别及食品安全的难题。试验以橄榄和其它6种常见油料为主,建立了PCR-CE-SSCP法鉴定橄榄油真伪的新方法。通过利用自行设计的uni4通用引物,用常规PCR对叶绿体上一段71bp大小基因进行扩增,并用基因分析仪分析得出7种油料的SSCP图谱,结果表明,采用PCR-CE-SSCP法能够区分橄榄及其它6种常见油料,可用于橄榄油真伪鉴别。 |
| 关 键 词： | 橄榄油鉴别 PCR-CE-SSCP 叶绿体基因 |
A New Method for Detecting Olive Oil Authentication-PCR-CE-SSCP Method |
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| Zhang Hailiang , Wu Yajun , Ju Xingrong , Wang Bin , Han Jianxun , Chen Ying.A New Method for Detecting Olive Oil Authentication-PCR-CE-SSCP Method[J].Journal of Chinese Institute of Food Science and Technology,2012,12(3):151-159. | |
| Authors: | Zhang Hailiang Wu Yajun Ju Xingrong Wang Bin Han Jianxun Chen Ying |
| Affiliation: | 1Institute of Food Science and Engineering,Nanjing Finance University,Nanjing 210003 2Chinese Academy of Inspection and Quarantine,Beijing 100123) |
| Abstract: | Olive oil authentication has always been a hard task for the food authentication and food safety.In this study,a new method for olive oil authentication was established-PCR-CE-SSCP method using olive oil and six common oils as test material.Short fragment(71bp) of the chloroplast gene was amplified by the routine polymerase chain reaction(PCR) and analyzed by single strand conformation polymorphism(SSCP) to get species-specific patterns.It showed that olive,rapeseed,corn,sunflower,soybean,peanut,and sesame all expressed specific SSCP patterns,and this PCR-CE-SSCP method distinctly can olive oil and other six common oils differentiate between and maybe used in olive oil authentication. |
| Keywords: | olive oil authentication PCR-CE-SSCP chloroplast gene |
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