| 高产抗生素Zwittermicin A苏云金芽孢杆菌A164发酵条件的优化 |
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| 引用本文： | 李洋,蒋泽军,刘红艳,李子院,郝再彬,宋福平,张杰.高产抗生素Zwittermicin A苏云金芽孢杆菌A164发酵条件的优化[J].青岛科技大学学报,2012(1):47-53. |
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| 作者姓名： | 李洋 蒋泽军 刘红艳 李子院 郝再彬 宋福平 张杰 |
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| 作者单位： | 桂林理工大学化学与生物工程学院;东北农业大学生命科学学院;中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室 |
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| 基金项目： | 国家重点基础研究发展计划973基金资助项目(2009CB118902);国家自然科学项目基金项目(31060193);桂林理工大学科研启动费项目(2009);广西自然科学基金项目(桂科回0731021) |
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| 摘 要： | 从土壤样品中筛选出高产抗生素(Zwittermicin A)的苏云金芽孢杆菌菌株:A164,对此菌种的发酵条件进行了探讨。以A164合成高含量抗生素为目标对发酵培养的碳源、氮源、时间等条件进行了单因素优化;采用Plackett-Burman设计,确定发酵条件的重要影响因子,根据爬坡路径法,确定了各个因子的最适范围,采用响应面分析法对因子再次进行优化,得到了最佳发酵条件。最佳发酵条件为:蛋白胨含量17.56g.L-1,葡萄糖20.0g.L-1,MgSO40.18g.L-1,CaCl20.08g.L-1,ZnSO40.20g.L-1,K2HPO41.3g.L-1,培养温度28.31℃,发酵时间36h。
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| 关 键 词： | 苏云金芽孢杆菌(Bt) 抗生素Zwittermicin A 发酵条件优化 |
Optimization of Fermentation Conditions of Bacillus Thuringiensis for High Production of Zwittermicin A |
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| LI Yang,JIANG Ze-jun,LIU Hong-yan,LI Zi-yuan,HAO Zai-bin,SONG Fu-ping,ZHANG Jie.Optimization of Fermentation Conditions of Bacillus Thuringiensis for High Production of Zwittermicin A[J].Journal of Qingdao University of Science and Technology:Natutral Science Edition,2012(1):47-53. |
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| Authors: | LI Yang JIANG Ze-jun LIU Hong-yan LI Zi-yuan HAO Zai-bin SONG Fu-ping ZHANG Jie |
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| Affiliation: | 1.Department of Chemical and Bioengineering,Guilin University of Technology,Guilin 541004,China; 2.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China; 3.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | Bacillus thuringiensis A164 for high production of Zwittermicin A was separated from the soil samples,then the fermentation conditions of this strain was discussed.According to single factorial experiments,carbon source,nitrogen source,and time were optimized.Plackett-Burman design was used to evaluate the importance of the related influential factor.Then the path of steepest ascent experiment was adopted to realize the optimal region of these importance factors.At last,the optimal fermentation conditions were obtained by response surface methodology as followed: 17.56 g·L-1 of soybean powder,20.0 g·L-1 of glucose,0.18 g·L-1 of MgSO4,0.08 g·L-1 of CaCl2,0.20 g·L-1 of ZnSO4,1.30 g·L-1 of K2HPO4,temperature 28.31 ℃,and fermentation time 36 h. |
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| Keywords: | Bacillus thuringiensis(Bt) antibiotic Zwittermicin A optimization of fermentation conditions |
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