榆树叶中类胡萝卜素的组成和含量

目的：分析榆树叶中类胡萝卜素主要组分的结构和含量。方法：丙酮提取榆树叶类胡萝卜素，采用高效液相色谱与二极管阵列检测器和质谱联用技术分析类胡萝卜素组分，依据其光谱和质谱信息并与标准品和文献相比较，确定主要类胡萝卜素的结构；采用高效液相色谱法测定不同采集时间的榆树叶中主要类胡萝卜素的含量。结果：叶黄素和β-胡萝卜素为榆树叶中主要的类胡萝卜素组分，其含量因采集时间不同而异：叶黄素含量范围为880.2～1 385.4 μg/g干样，平均含量为1 098.3 μg/g干样；β-胡萝卜素含量范围为142.8～364.2 μg/g干样，平均含量为297.7 μg/g干样。结论：榆树叶有望成为叶黄素开发的食品新资源。

Identification and Quantification of Carotenoids in Leaves of Ulmus pumila L.

Objective: To determine the structures and contents of major carotenoids in leaves of elm (Ulmus pumila L.)
collected at different time points during one year. Methods: Carotenoids from elm leaves were extracted using acetone. The
major carotenoids were analyzed with the use of high performance liquid chromatography with diode array detection coupled
to mass spectrometry (HPLC-DAD-MS) and identified by comparison of UV-visible and mass spectral data with reference
standards or data reported in the literature. The contents of major carotenoids were measured by HPLC. Results: Two major
carotenoids were identified as lutein and β-carotene. The level ranged from 880.2 to 1 385.4 μg/g (dry weight) for lutein
and from 142.8 to 364.2 μg/g (dry weight) for β-carotene with an average content of 1 098.3 and 297.7 μg/g (dry weight),
respectively. Conclusion: Ulmus pumila L. is a new resource of lutein.