| 人乳铁蛋白在原核中的融合表达 |
| |
| 引用本文： | 李树芹,章立群,孙雪梅,贾士乾,周伟明,张建华,吕加平,张列兵. 人乳铁蛋白在原核中的融合表达[J]. 中国乳品工业, 2002, 30(3): 3-5 |
| |
| 作者姓名： | 李树芹 章立群 孙雪梅 贾士乾 周伟明 张建华 吕加平 张列兵 |
| |
| 作者单位： | 北京三元食品股份有限公司,北京,102206 |
| |
| 摘 要： | 设计了特异引物，通过PCR扩增人乳铁蛋白（HLF）基因，将扩增的DNA片段重组到原核表达载体pGEX-4T3中，重组体转化大肠杆菌BL21菌株，经IPTG诱导后，得到了高效融合表达。分析结果表明，获得了特异表达的乳铁蛋白。
|
| 关 键 词： | 人乳 铁蛋白 PCR 融合表达 大肠杆菌BL21菌株 |
| 文章编号： | 1001-2230(2002)03-0003-03 |
| 修稿时间： | 2002-02-27 |
Cloning and fusion expression of human lactoferrin protein gene in prodaryotic expression vector |
| |
| LI Shu qin,ZHANG Li qun,SUN Xue mei,JIA Shi qian,ZHOU Wei ming,ZHANG Jian hua,LU Jia ping,ZHANG Lie bing. Cloning and fusion expression of human lactoferrin protein gene in prodaryotic expression vector[J]. China Dairy Industry, 2002, 30(3): 3-5 |
| |
| Authors: | LI Shu qin ZHANG Li qun SUN Xue mei JIA Shi qian ZHOU Wei ming ZHANG Jian hua LU Jia ping ZHANG Lie bing |
| |
| Abstract: | By designing special,primers,human lactoferrin protein gene was amplified by PCR and cloned into pGEX-4T3 vector,then transformed it into escherichia coli BL21.Induced by IPTG,the fusion protein was expressed specially.The result of western blot analysis indicated that the fusion protein was GST-human lactoferrin protein. |
| |
| Keywords: | human lactoferrin protein PCR fusion expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
