Droplet microfluidic preparation of au nanoparticles-coated chitosan microbeads for flow-through surface-enhanced Raman scattering detection |
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Authors: | Wei Wang Chun Yang Xiao Qiang Cui Qiao Liang Bao Chang Ming Li |
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Affiliation: | (1) School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore, 637457, Singapore;(2) School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 637457, Singapore;(3) Present address: Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda Osaka, 563-8577, Japan;(4) Present address: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 119077, Singapore; |
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Abstract: | An integrated microfluidic device was fabricated to enable on-chip droplet forming, trapping, fusing, shrinking, reaction
and producing functional microbeads for a flow-through single bead-based molecule detection. Dielectrophoresis (DEP) force
was used to transport target polymer droplets into different predefined microwells, where the droplets were fused through
electrocoalescence to form a new one with a desired diameter. In a continuous water loss process with water diffusion to oil
phase, the polymer droplet was shrunken and solidified to form a polymer microbead. For a demonstration, Au nanoparticles-coated
chitosan microbeads were in situ fabricated through droplet trapping, fusion and shrinking, followed by synthesis of Au nanoparticles
on the microbead surface via a photoreduction process. The produced Au nanoparticle/chitosan microbead embedded in the microwell
resulted in a highly sensitive, flow-through surface-enhanced Raman scattering (SERS) detection of Rhodamine 6G (R6G). This
work successfully demonstrates an integrated droplet based lab-on-a chip and its application to fabricate an extremely high-throughput
single bead based detection platform. |
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