Effects of Fe and Mn Deficiencies on the Root Protein Profiles of Tomato (Solanum lycopersicum) Using Two-Dimensional Electrophoresis and Label-Free Shotgun Analyses |
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| Authors: | Laura Ceballos-Laita Daisuke Takahashi Matsuo Uemura Javier Abadía Ana Flor Lpez-Milln Jorge Rodríguez-Celma |
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| Affiliation: | 1.Plant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, 50059 Zaragoza, Spain; (L.C.-L.); (J.A.); (A.F.L.-M.);2.United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan; (D.T.); (M.U.);3.Department of Plant-Bioscience, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan |
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| Abstract: | Iron (Fe) and manganese (Mn) are two essential elements for plants that compete for the same uptake transporters and show conflicting interactions at the regulatory level. In order to understand the differential response to both metal deficiencies in plants, two proteomic techniques (two-dimensional gel electrophoresis and label-free shotgun) were used to study the proteome profiles of roots from tomato plants grown under Fe or Mn deficiency. A total of 119 proteins changing in relative abundance were confidently quantified and identified, including 35 and 91 in the cases of Fe deficiency and Mn deficiency, respectively, with 7 of them changing in both deficiencies. The identified proteins were categorized according to function, and GO-enrichment analysis was performed. Data showed that both deficiencies provoked a common and intense cell wall remodelling. However, the response observed for Fe and Mn deficiencies differed greatly in relation to oxidative stress, coumarin production, protein, nitrogen, and energy metabolism. |
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| Keywords: | root shotgun proteomics Mn deficiency Fe deficiency tomato 2-DE |
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