多样品同时萃取-气相色谱法测定食品中山梨酸和苯甲酸

研究了多样品同时萃取/气相色谱法对食品中山梨酸和苯甲酸的快速测定。以醋酸乙酯+正己烷(1+1)混合溶剂对样品和标准系列进行一次性萃取,用SE-30宽口径毛细柱作分析柱对萃取相进行分析。方法条件下,样品中山梨酸和苯甲酸的检出限为1mg/kg,线性范围25-500 mg/kg,回收率93.2%、102.4%,相对标准偏差(RSD)为1.9-4.1%。方法操作简便快捷易于控制,使用试剂少成本低,用于批量样品的山梨酸和苯甲酸快速测定获得良好效果。

Determination of Sorbic Acid and Benzoic Acid in Foods Using Multi-sample Simultaneous Extraction-gas Chromatography

A novel method for determination of sorbic acid and benzoic acid in food was developed using multi-sample simultaneous extraction-gas chromatography. The samples and standard series solution were extracted simultaneously, with a mixture of ethyl acetate and hexane (1+1) as extraction solvent. The contents of sorbic acid and benzoic acid were measured by SE-30 wide caliber capillary. In the selected conditions, the detection limit for sorbic acid and benzoic acid was 1 mg/kg, the linear range was 25~500 mg/kg, the recoveries was 93.2~102.4% and the RSD was 1.9~4.1%. The method was simple, rapid, easy control and low-cost. It can be used for the rapid quantitative analysis of batch sample.