| 酶转化人参皂苷C-K的提取工艺 |
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| 引用本文： | 李翠翠,刘廷强,王颖,鱼红闪,金凤燮. 酶转化人参皂苷C-K的提取工艺[J]. 大连轻工业学院学报, 2009, 28(2): 87-89 |
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| 作者姓名： | 李翠翠 刘廷强 王颖 鱼红闪 金凤燮 |
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| 作者单位： | 大连工业大学生物与食品工程学院; |
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| 基金项目： | 国家自然科学基金资助项目(30470055);;辽宁省教育厅创新团队项目(2007T006) |
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| 摘 要： | 以粗品人参皂苷C-K为原料,进行脱色、脱脂、硅胶柱层析法分离纯化,得到纯度较高的人参皂苷C-K。结果表明,粗品人参皂苷C-K样品利用D-296树脂脱色柱脱色,除去色素占总质量的4.74%,石油醚脱脂共除去杂质占总质量的7.46%;应用硅胶柱法分离得到较纯人参皂苷C-K得率为41.2%;样品结晶后收率为90%,高效液相色谱检测结晶产物中稀有人参皂苷C-K的质量分数为98.09%。
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| 关 键 词： | 人参皂苷 薄层层析 硅胶柱层析 高效液相色谱 |
Extraction of ginsenoside Compound-K |
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| LI Cui-cui,LIU Ting-qiang,WANG Ying,YU Hong-shan,JIN Feng-xieSchool of Biological , Food Engineering,Dalian Polytechnic University,Dalian,China. Extraction of ginsenoside Compound-K[J]. Journal of Dalian Institute of Light Industry, 2009, 28(2): 87-89 |
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| Authors: | LI Cui-cui LIU Ting-qiang WANG Ying YU Hong-shan JIN Feng-xieSchool of Biological & Food Engineering Dalian Polytechnic University Dalian China |
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| Affiliation: | LI Cui-cui,LIU Ting-qiang,WANG Ying,YU Hong-shan,JIN Feng-xieSchool of Biological & Food Engineering,Dalian Polytechnic University,Dalian116034,China |
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| Abstract: | The ginsenoside Compound-K(C-K) was isolated and purified from crude C-K by resin decolorizing,petroleum benzin ungreasing,silica gel column chromatography and crystal.4.74% impurity was removed by resin D-296 and 7.46% impurity was removed by petroleum benzin.A 41.2% C-K was obtained after isolation on silica gel.After crystallization the C-K yield was 90%,the purity of which was 98.09% by HPLC detection. |
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| Keywords: | ginsenoside thin-layer chromatography silica gel column HPLC |
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