Three-dimensional reconstruction of single particles negatively stained or in vitreous ice. |
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Authors: | J Frank M Radermacher |
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Affiliation: | Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509. |
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Abstract: | The random-conical reconstruction method has been highly successful in three-dimensional imaging of macromolecules under low-dose conditions. This article summarizes the different steps of this technique as applied to molecules prepared with negative staining or vitreous ice, and sketches out the current directions of development. We anticipate that by using new instrumental developments, transfer function correction and computational refinement techniques, a resolution in the range of 7-10 A could ultimately be achieved. |
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