微振动应力环境影响羟基磷灰石陶瓷生物活性及力学稳定性的体外评价

本研究考察微振动应力环境(MVS)下羟基磷灰石(HA)陶瓷的生物活性及力学稳定性, 探讨体内生理应力环境对HA陶瓷骨诱导性的影响。扫描电子显微镜(SEM)和钙离子释放结果均显示, MVS有利于具有较高微孔隙率((29±1.2)%, (26.4±0.3)%)HA材料的钙离子再沉积和类骨磷灰石层形成, 而对于微孔隙率较低((10.6±0.8)%)的HA材料则促进其钙离子释放。蛋白吸附结果显示40 Hz的MVS促进了HA的蛋白吸附, 60 Hz的MVS则显著抑制HA的蛋白吸附行为。抗压试验表明MVS应力环境并未影响HA多孔支架的力学稳定性。研究表明应力环境是影响HA陶瓷生物活性和骨诱导性的重要因素。

Bioactivity and Mechanical Stability of Hydroxyapatite Ceramicsunder Micro-vibration Environment

This study investigated the bioactivity and mechanical stability of hydroxyapatite (HA) ceramics under micro-vibration stress environment (MVS), and explored influence of physiological stress environment on osteoinductivity of HA ceramics. Results of scanning electron microscopy (SEM) and calcium ion release showed that MVS was beneficial for calcium ion re-deposition and bone-like apatite layer formation in HA with high micro-porosity ((29±1.2)%, (26.4±0.3)%, respectively), while calcium ion release of HA materials with a lower micro-porosity ((10.6±0.8)%) was promoted. Protein adsorption results displayed that 40 Hz MVS promoted while 60 Hz MVS significantly inhibited HA protein adsorption behavior. The compression tests demonstrated that stress environment of MVS did not affect the mechanical stability of the HA scaffolds. Our results suggested that stress environment is an important factor affecting the bioactivity and osteoinductivity of HA ceramics.