邻二氮菲-Fe~(2+)法测定牦牛乳酪蛋白酶解产物的抗氧化性

采用邻二氮菲-Fe2+法测定牦牛乳酪蛋白酶解产物的抗氧化性。通过单因素分析,确定了试剂的用量及反应条件对样品吸光度及羟自由基清除率的影响。优化得到的反应条件为:1 mL 1.5 mmol/L邻二氮菲,2 mLpH7.4磷酸盐缓冲液,1 mL抗氧化剂,充分摇匀后,加入1 mL 0.5 mmol/L FeSO4溶液,混匀,加1 mL 0.1%H2O2,具塞于38℃反应50 min,于波长536 nm处测定其吸光度。结果表明,改良的邻二氮菲-Fe2+法测定结果重现性好,适于酪蛋白酶解产物抗氧化性的测定。

Determination of Antioxidant Activity of Enzymatic Hydrolysate of Yak Casein by Phenanthroline-Fe2+ Method

Antioxidant activity of enzymatic hydrolysate of yak casein was investigated by phenanthroline-Fe2+ method.By univariate analysis,effects of the reagent dosage and reaction condition on absorbance and scavenging rate of hydroxyl free radicals were investigated.The optimal reaction conditions were as followes: 1 mL of 1.5 mmol/L Phenanthroline,2 mL of pH7.4 phosphate buffer and 1mL antioxidants was successively collected in a test tube with stopper and mixed fully,then 1 mL of 0.5 mmol/L ferrous sulfate solution was added and mixed,later 1mL of 0.1% hydrogen peroxide was added.Absorbance of sample was detected at 536 nm using ultraviolet spectrophotometer after reaction at 38℃ for 50 min.The results showed that improved method is reproducible to results of determination and applicable to determination of antioxidant activity of hydrolysate of casein.