超声辅助酶法提取河蚬多糖

选择超声辅助酶法提取河蚬多糖,确定了不同超声时间、超声功率、料水比、酶解时间等因素对河蚬多糖提取率的影响,并进行了正交试验分析。结果表明,最佳提取工艺条件为:超声时间40 min、超声功率80W、料液比1∶30、酶解时间90 min,在此条件下,河蚬多糖的提取率为4.86%。

Study on Ultrasonic-assisted Enzymolysis Extraction Polysaccharides from Corbicula fluminea

Using Corbicula fluminea as raw material,the content of polysaccharides was determined by the phenol-sulphate acid method.On the basis of single factors tests,the effects of the four factors including ultrasonic time,solid-liquid ratio,digestion time and ultrasonic power on the yield of polysaccharides from Corbicula fluminea were studied through orthogonal experiment design.The results showed that the decreasing order of effect of the four factors on extraction yield of polysaccharides were as follow: ultrasonic time,solid-liquid ratio,digestion time,and ultrasonic power,in which ultrasonic time had significant effect on the yield of polysaccharides(P< 0.1).And solid-liquid ratio,digestion time and ultrasonic power had no significant effect on the yield of polysaccharides.The optimum parameters were as follows: ultrasonic time of 40 min,ultrasonic power of 80 W,solid-liquid ratio of 1∶ 30 and digestion time of 90 min.The yield of polysaccharides reached 4.86% using the optimum experiment parameters.