| 基于玉米黄浆及淀粉培养的罗耳阿太菌发酵多糖工艺研究 |
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| 引用本文： | 苗琇岩,李鸿梅,魏明,赵慧,闵伟红,马艳秋.基于玉米黄浆及淀粉培养的罗耳阿太菌发酵多糖工艺研究[J].食品科学,2014,35(19):194-202. |
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| 作者姓名： | 苗琇岩 李鸿梅 魏明 赵慧 闵伟红 马艳秋 |
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| 作者单位： | 1.吉林农业大学食品科学与工程学院，吉林 长春 130118；2.吉林农业大学图书馆，吉林 长春 130118 |
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| 基金项目： | 吉林省科技发展计划项目 |
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| 摘 要： | 从君子兰（Clivia miniata Regel）上筛选出一株罗耳阿太菌（Athelia rolfsii）AY6657741。通过响应面分析试验确定其较适发酵条件为：培养温度29 ℃、pH 4.55、接种量7%、转速200 r/min、发酵培养5.5 d；较适培养基成分为：玉米淀粉35 g/L、玉米黄浆50 mL/L、NaNO3 3.1 g/L、KH2PO4 1.0 g/L、KCl 0.5 g/L、MgSO4?7H2O 0.25 g/L、柠檬酸1.4 g/L，结果表明，在最适条件下菌株粗多糖产量达16.135 g/L。
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| 关 键 词： | 罗耳阿太菌 玉米黄浆 玉米淀粉 优化工艺 粗多糖 |
Optimization of Culture Conditions for the Production of Polysaccharides by Athelia rolfsii in a Medium Consisting of Corn Steep Liquor and Starch |
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| MIAO Xiu-yan,LI Hong-mei,WEI Ming,ZHAO Hui,MIN Wei-hong,MA Yan-qiu.Optimization of Culture Conditions for the Production of Polysaccharides by Athelia rolfsii in a Medium Consisting of Corn Steep Liquor and Starch[J].Food Science,2014,35(19):194-202. |
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| Authors: | MIAO Xiu-yan LI Hong-mei WEI Ming ZHAO Hui MIN Wei-hong MA Yan-qiu |
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| Affiliation: | 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;
2. Library of Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | This study was intended to employ response surface methodology to establish the optimum culture conditions
and medium components for the production of polysaccharides by Athelia rolfsii AY6657741 isolated from Clivia miniata
Regel. When Athelia rolfsii AY6657741 was inoculated at an inoculum size of 7% by volume to a culture medium containing
35 g/L corn starch, 50 mL/L corn steep liquor, 3.1 g/L NaNO3, 1.0 g/L KH2PO4, 0.5 g/L KCl, 0.25 g/L MgSO4·7H2O,
and 1.4 g/L citric acid (pH 4.55) and cultivated at 29 ℃ with a rotation speed of 200 r/min for 5.5 d, 16.135 g/L crude
polysaccharides were produced. |
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| Keywords: | Athelia rolfsii corn steep liquor corn starch optimal culture conditions crude polysaccharides |
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