| 小麦秸秆酶法制备低聚木糖及其抗氧化活性 |
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| 引用本文： | 田龙,王云,马晓建.小麦秸秆酶法制备低聚木糖及其抗氧化活性[J].食品科学,2014,35(20):88-92. |
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| 作者姓名： | 田龙 王云 马晓建 |
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| 作者单位： | 1.南阳师范学院生命科学与技术学院，河南 南阳 473061；2.郑州大学化工与能源学院，河南 郑州 450001 |
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| 摘 要： | 研究小麦秸秆酶法制备低聚木糖的最佳工艺及其抗氧化作用。确定低聚木糖的最佳制备条件为：酶解pH 6.0、木聚糖酶用量3 g/L、底物质量浓度70 g/L、酶解温度50 ℃、酶解时间4 h。在此条件下，酶解产生的还原糖含量为9.5 g/L，可溶性总糖含量为26.3 g/L，平均聚合度为2.77。考察低聚木糖的抗氧化活性，发现其对?OH、O2－?和DPPH自由基的清除能力呈量效关系。
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| 关 键 词： | 小麦秸秆 低聚木糖 抗氧化活性 |
Preparation and Antioxidant Activity of Xylooligosaccharides from Wheat Straw by Enzymatic Hydrolysis with Xylanase |
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| TIAN Long,WANG Yun,MA Xiao-jian.Preparation and Antioxidant Activity of Xylooligosaccharides from Wheat Straw by Enzymatic Hydrolysis with Xylanase[J].Food Science,2014,35(20):88-92. |
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| Authors: | TIAN Long WANG Yun MA Xiao-jian |
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| Affiliation: | 1. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China;
2. College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China |
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| Abstract: | The enzymatic hydrolysis of wheat straw for preparing xylooligosaccharides using xylanase was optimized using
an orthogonal array design, and the xylooligosaccharides obtained were assessed for antioxidant activity. A hydrolysis
temperature and 4 h hydrolysis at an enzyme concentration of 3 g/L and a substrate concentration of 70 g/L with an
initial pH of 6.0 proved optimal for the preparation of xylooligosaccharides. The optimized conditions yielded 9.5 g/L
of reducing sugar and 26.3 g/L of soluble sugar in the product with an average degree of polymerization of 2.77. The
xylooligosaccharides showed dose-dependent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH),
hydroxyl and superoxide anion radicals. |
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| Keywords: | wheat straw xylooligosaccharides antioxidant activity |
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