植物乳杆菌NCU116对高脂饮食大鼠肾脏的保护作用

目的：探讨植物乳杆菌NCU116对高脂饮食大鼠肾功能及抗氧化能力的影响。方法：40 只健康雄性Sprague-Dawley（SD）大鼠随机分为正常对照组、高脂模型对照组和植物乳杆菌NCU116低（108 CFU/mL）、高（109 CFU/mL）剂量组，其中正常对照组以普通饲料喂养，其余各组在高脂饲料喂养的同时结合植物乳杆菌NCU116进行干预。通过分析肾脏系数，检测血液中血肌酐（serum creatinine，Scr）、尿素氮（blood ureanitrogen，BUN）和尿酸（uric acid，UA）水平，并测定肾脏中超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、总抗氧化能力（total antioxidative capacity，T-AOC）、过氧化氢酶（catalase，CAT）活性和丙二醛（malondialdehyde，MDA）水平，病理学观察肾脏病变情况，研究植物乳杆菌NCU116对高脂饮食大鼠肾脏的保护作用。结果：高剂量植物乳杆菌NCU116对大鼠UA、SOD、GSH-Px、CAT、T-AOC和MDA等指标及肾脏病变具有显著性改善作用（P＜0.05）。结论：植物乳杆菌NCU116对高脂饮食大鼠肾脏氧化损伤、肾功能有一定的保护作用。

Protective Effect of Lactobacillus plantarum NCU116 on Renal Injury in Rats Fed a High-Fat Diet

Aim: To investigate the effect of Lactobacillus plantarum NCU116 (L. plantarum NCU116) on antioxidant
activity and renal function in rats fed a high-fat diet. Methods: Totally 40 male rats were randomly divided into 4 groups:
L. plantarum NCU116 groups with two doses (108 and 109 CFU/mL), high-fat model group and normal control group. After
5-week administration, the renal index was analyzed, and the contents of serum creatinine (Scr), blood urea nitrogen (BUN),
and uric acid (UA) were determined. Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase
(GSH-Px), and catalase (CAT), total antioxidant capacity (T-AOC) and the content of malondialdehyde (MDA) in renal
homogenates were determined. The kidney was examined histopathologically as well. Results: Compared with highfat
model group, the levels of UA, SOD, GSH-Px, CAT, T-AOC, and MDA in the high-dosage L. plantarum NCU116
group were obviously improved (P ＜ 0.05), and these biochemical observations were supplemented by histopathological
examination of the kidney. Conclusion: L. plantarum NCU116 can protect the kidney from oxidative stress and improve the
renal function of high-fat diet fed rats.