荧光光谱法研究桑色素与人血清白蛋白的相互作用

采用荧光猝灭光谱、紫外-可见吸收光谱研究了桑色素与人血清白蛋白(HSA)的结合作用。实验表明桑色素对HSA的荧光猝灭属于单一静态猝灭反应,在溶液中以摩尔比1:1牢固结合,各结合反应的平衡常数Kp>105,结合常数Kb>104;根据F rster非辐射能量转移机理,求算HSA与桑色素间距离r为3.81~3.58nm,能量转移效率E为0.18~0.13。并根据结合反应的热力学常数推测了药物与HSA之间的主要作用力类型为疏水作用力和偶极-偶极作用力。

Study on the Interaction of Morin with Human Serum Albumin by Fluorescence Spectroscopic Methods

The interaction of Morin with Human Serum Albumin （HSA） was studied by Spectrometry , UV- visible absorption spectrophotometry and fluorescence spectrometry. The result show that the Morin could induce an endogenous fluorseence quenching of HSA ,which was proved to be a process of static quenching by the fitting equations of Stern- Volmer and double logarithm equation. The binding constants were determined to be more than 104.The binding site （n） was about 1.The binding distance was estimated to be 3.81-3.58nm according to Ffirster nonradiation energy transfer mechanism.Energy transfer effciency of E were 0.18N0.13J.The thermodynamic parameters suggested that the interaction forces between Morin and HSA were mainly hydrophobic interaction and dipole-dipole interaction.