土壤中矮壮素、缩节胺的残留对番茄酱品质的影响

采用HPLC-MS/MS技术,同时测定土壤中的矮壮素和缩节胺残留。土壤样品经缓冲溶液提取后,经Agilent ZORBAX Rx-Si(l100mm×3.0mm,1.8μm)色谱柱分离,以含浓度10mmol/L NH4Ac+0.2%HCOOH的水溶液和甲醇(60:40,V/V)为流动相进行等度洗脱,经正离子多反应监测模式测定目标化合物。结果表明:两种化合物在1.0-50.0μg/L浓度范围内线性关系良好,定量限(LOQ)矮壮素为0.8μg/L,缩节胺为0.6μg/L;加标回收率平均在97.6%-99.5%;相对标准偏差(RSD)平均在2.5%-2.9%,适用于检测土壤中矮壮素和缩节胺。

Effect of Chlormequat and Mmepiquat Chloride Residues in Soil on Quality of Tomato Paste

The residues of chlormequat and mepiquat chloride residues in soil were studied by means of high performance liquid chromatography- tandem mass spectrometer.The samples were extraction with water and cleaned up on 50%acetonitrile. The target compounds were separated on an Agilent ZORBAX Rx- Sil （100mm × 3.0mm,1.8μm） chromatographic column by equivalently elution using 10mmol/L ammonium acetate-0.2% formic acid solution and methanol（60：40,V/V）as mobile phase. Determination of the target compounds by positive ion multiple reaction monitoring mode.The results show that the two kinds of compound has a well linear relationship among the range of 1.0-50.0 u g/L;Limits Of Quantification （LOQ）：CCC was 0.8μg/L,DPC was 0.6μg/L; the recovery rates were 97.6%-99.5%; relative standard deviation were 2.5%-2.9%,it is suitable for residues determination of MQ and CQ in soil.