| 色氨酸在自制人血清白蛋白手性柱上的拆分及手性识别机理研究 |
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| 引用本文： | 林艳萍,亢珊珊,姚碧霞,翁文.色氨酸在自制人血清白蛋白手性柱上的拆分及手性识别机理研究[J].福建分析测试,2016(2):7-10. |
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| 作者姓名： | 林艳萍 亢珊珊 姚碧霞 翁文 |
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| 作者单位： | 闽南师范大学化学与环境学院,福建 漳州,363000 |
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| 基金项目： | 福建省自然科学基金(2012J06005)，福建省质量技术监督局项目(fjqi2013109)资助。 |
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| 摘 要： | 人血清白蛋白与三氯聚氰活化的氨丙基硅胶反应,制得人血清白蛋白键合手性固定相。反相模式下,色氨酸在该手性固定相上获得理想的拆分,分离因子可达3.51,分离度达5.49。探讨了流动相p H值、有机修饰剂、柱温等对手性拆分的影响。通过前沿分析法对色谱保留机理进行了探讨。
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| 关 键 词： | 手性固定相 手性拆分 人血清白蛋白 色氨酸 前沿分析法 |
Enantioseparation of Tryptophan on Human Serum Albumin-immobilized Chiral Stationary Phase and Study on the Mechanism of Chiral Recognition |
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| Abstract: | Human serum albumin(HSA)based chiral stationary phase(CSP)was conveniently prepared by HSA and 2,4, 6-trichloro-1,3,5-triazine activated aminopropyl silica gel. Tryptophan was enantioseparated successfully on the as-pre?pared CSP under the reverse-phase mode. The separation factor and the resolution reached 3.51 and 5.49,respectively. The effects of pH value of mobile phase,type and content of organic modifier,and column temperature on the enantiosepa?ration of tryptophan were investigated. The mechanism of chiral recognition was studied further with frontal analysis method. |
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| Keywords: | Chiral stationary phase Enantioseparation HSA Tryptophan Frontal analysis |
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