|
|||||
|
|
Results of an interlaboratory assessment of a screening method of genetically modified organisms in soy beans and maize |
|
| Authors: | M. Lipp E. Anklam P. Brodmann K. Pietsch J. Pauwels |
| Affiliation: | a Commission of the European Union, Joint Research Center, Institute for Health and Consumer Protection, Food Products Unit, I-21020, Ispra (Va), Italy b Kantonales Labor, Kannenfeldstrassse 2, 4012, Basel, Switzerland c Chemische Landesuntersuchungsanstalt, Bissierstr. 5, 79114, Freiburg, Germany d European Commission, Joint Research Center, Institute of Reference Materials and Measurements, Retieseweg, 2440, Geel, Belgium |
| Abstract: | The preliminary results on an interlaboratory trial on the detection of genetically modified organisms (GMO) are presented. The method applied is based on the detection of modified DNA using the polymerase chain reaction (PCR) for amplification. The amplified fragments analysed are derived from the 35S promotor and the NOS terminator used for modification and are present in 26 from the 28 GMOs currently already approved or under approval by the competent authorities. This method fits as a screening method indicating the presence of GMO in food. However, it does not allow an identification of the kind of GMO present in the samples. Samples of soybean and maize flour containing 0%, 0.1%, 0.5% and 2% GMO had been prepared for this study and are also already commercially available. In this paper the combined results from 27 laboratories are presented, indicating that on average the probability of false positive or false negative results is only about 1% for soybeans and below 5% for maize. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |