Temperature-gradient gel electrophoresis as a screening tool for polymorphisms in multigene families |
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Authors: | C Schl?tterer |
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Affiliation: | Zoologisches Institut der Universit?t München, Germany. |
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Abstract: | Information about sequence variability between different copies of a multigene family is indispensable for understanding the evolutionary mechanisms acting on multigene families. However, their high copy number has been a major obstacle to systematic analysis. Exemplified by the internal transcribed spacer 1 (ITS1) of the rDNA in Drosophila melanogaster, it is shown how temperature gradient gel electrophoresis (TGGE) can be used to study sequence polymorphisms in a multigene family. Experimental conditions influencing the melting behavior of the ITS1 fragment are discussed as well as discrepancies between observed and calculated melting patterns. |
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