龙眼核中的神经酰胺和脑苷脂的分离和鉴定

研究龙眼(Dimocarpus longan Lour.)果核的化学成分,采用硅胶柱色谱对龙眼果核石油醚提取物和体积分数95%乙醇提取物进行分离,并利用理化性质和光谱数据鉴定化合物的结构。从龙眼果核的提取物中分离得到一组混合神经酰胺(化合物1、2)和一组混合脑苷脂(化合物3～6),结构鉴定为Rel-(3S,4S,5S)-3-[(2'R)-2'-羟基二十二酰胺]-4-羟基-5-[(4″Z)-十四烷-4″-烯]-2,3,4,5-四氢呋喃(1)、Rel-(3S,4S,5S)-3-[(2'R)-2'-羟基二十四酰胺]-4-羟基-5-[(4″Z)-十四烷-4″-烯]-2,3,4,5-四氢呋喃(2)、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8E)-2-(2'-羟基二十四酰胺)-8-十八烯-1,3,4-三醇(3)、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8Z)-2-(2'-羟基二十四酰胺)-8-十八烯-1,3,4-三醇(4)、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8E)-2-(2'-羟基二十二酰胺)-8-十八烯-1,3,4-三醇(5)、1-O-(β-D-吡喃葡萄糖基)-(2S,3S,4R,8Z)-2-(2'-羟基二十二酰胺)-8-十八烯-1,3,4-三醇(6)。

Isolation and identification of ceramides and cerebrosides from longan (Dimocarpus longan Lour.) seeds

To investigate the chemical constituents of longan(Dimocarpus longan Lour.) seeds,a ceramide molecular species(compounds 1 & 2) were isolated from petroleum ether extract and a cerebroside molecular species(compounds 3～6) from the φ(ethanol)=95% extract from longan seeds by silica gel column chromatography,their structures were identified on the basis of physical and chemical properties and spectral analysis.Compounds 1 & 2 and compounds 3～6 were isolated and identified:Rel-(3S,4S,5S)-3-[(2′R)-2′-hydroxydocosylamino]-4-hydroxy-5-[(4″Z)-tetradecane-4″-ene]-2,3,4,5-tetrahydrofuran(1),Rel-(3S,4S,5S)-3-[(2′R)-2′-hydroxytetracosanoylamino]-4-hydroxy-5-[(4″Z)-tetradecane-4″-ene]-2,3,4,5-tetrahydrofuran(2),1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-(2′-hydroxylignoceroylamino)-8-octadecene-1,3,4-triol(3),1-O-β-D-glucopyranosyl-(2S,3S,4R,8Z)-2-(2′-hydroxylignoceroylamino)-8-octadecene-1,3,4-triol(4),1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-(2′-hydroxyldocosylamino)-8-octadecene-1,3,4-triol(5),1-O-β-D-glucopyranosyl-(2S,3S,4R,8Z)-2-(2′-hydroxydocosylamino)-8-octadecene-1,3,4-triol(6).