Analysis of triacylglyceride hydroperoxides in vegetable oils by nonaqueous reversed-phase high-performance liquid chromatography with ultraviolet detection |
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Authors: | Christina Bauer-Plank Lisette Steenhorst-Slikkerveer |
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Affiliation: | (1) Unilever Research Vlaardingen, Olivier van Noortlaan 120, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands |
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Abstract: | Triacylglyceride hydroperoxides (HPO-TAG), the primary autoxidation products of triacylglycerides (TAG), have been analyzed
in polyunsaturated vegetable oils by means of nonaqueous reversed-phase high-performance liquid chromatography (HPLC) with
ultraviolet detection. Using a retention time model based on equivalent carbon numbers, mono- and bishydroperoxy TAG and hydroxy
TAG could be identified. The correlation between the peroxide value (POV) determined by iodometric titration and quantitative
HPLC results for HPO-TAG was established for sunflower oil samples with POV between 0.5 and 50 meq/kg. The recovery of HPO-TAG
in the HPLC procedure was found to be close to 100% in the POV range of 4 to 71 meq/kg. Absolute quantitative results for
HPO-TAG in sunflower oil samples could not be obtained accurately, as molar extinction coefficients of HPO-TAG occurring in
natural oils deviate from those of available HPO-TAG reference compounds. |
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Keywords: | Autoxidation nonaqueous reversed-phase HPLC sunflower oil triacylglyceride hydroperoxides triacylglycerols |
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