Synthesis of polyunsaturated fatty acid-enriched triglycerides by lipase-catalyzed esterification |
| |
Authors: | L Esteban Cerdán A Robles Medina A Giménez Giménez M J Ibáñez González E Molina Grima |
| |
Affiliation: | (1) Departamento de Ingeniería Química, Universidad de Almería, E-04071 Almería, Spain |
| |
Abstract: | This paper reports on the synthesis of triglycerides by enzymatic esterification of polyunsaturated fatty acids (PUFA) with
glycerol. A PUFA concentrate obtained from cod liver oil was used to optimize the reaction to favor triglyceride synthesis
with lipases. The type and amount of lipase and organic solvent, glycerol content, temperature, water content, and amount
and time of addition of molecular sieves were studied. The optimal reaction mixture and conditions were: 9 mL hexane, 60°C,
0.5% (vol/vol) water, 1 g molecular sieves added after 24 h of reaction, glycerol/fatty acid molar ratio 1:3 and 100 mg of
Novozym 435 (Novo Nordisk A/S) lipase. Under these conditions, an enriched triglyceride yiedl of 84.7% containing 27.4% eicosapentaenoic
acid and 45.1% docosahexaenoic acid was obtained from a cod liver oil PUFA concentrate. |
| |
Keywords: | Docosahexaenoic acid (DHA) eicosapentaenoic acid (EPA) lipase-catalyzed esterification polyunsaturated fatty acids (PUFA) triglyceride synthesis |
本文献已被 SpringerLink 等数据库收录! |
|