云南普洱茶多糖提取工艺及翻堆样中含量测定的研究

以水为溶剂,用无水乙醇做沉淀剂提取茶多糖,研究了云南普洱茶茶多糖提取最佳方法并测定不同翻堆样普洱茶(人工接种优势菌种,“渥堆”封闭发酵,10d翻堆一次,40d结束)中茶多糖含量。试验证明:最佳提取工艺为无水乙醇浸提4h,取茶渣,沸水提取40min,重复3次,2倍体积无水乙醇沉淀;“渥堆”是普洱茶制作过程中的一道特殊工序,是形成普洱茶品质特征最关键的一步,翻堆样中茶叶渥堆时间越长,茶多糖含量越高,一翻堆含量仅0.45%,到四翻堆上升到1.68%,以上均为干物质计。

Study on tea polysaccharides extraction methods and content on different piles of Yunnan Pu-erh tea

Tea polysaccharides was extracted from Yunnan Pu-erh tea using distilled water as solution and ethanol as precipitator.Results showed that the optimum extraction conditions are that extract with ethanol for four hours,soak the remains with boiled water for 40min,repeat 3 times,sedimentate with 2 times volume ethanol;the content of tea polysaccharides is increasing with the fermentation time in different piles of Yunnan Pu-erh tea,0.45% tea polysaccharides obtained in the first pile,and 1.68% in the forth pile.