橡子粉同步液化糖化产燃料乙醇的发酵条件优化

以除去单宁的橡子粉为原料,应用活性干酵母同步液化糖化发酵(SLSF)制备燃料乙醇,并通过单因素试验和正交试验优化发酵条件。结果表明,同步液化糖化发酵技术适用于橡子粉发酵制备燃料乙醇;发酵的最佳条件为:除去单宁的橡子粉20 g,料液比为1:3(g:mL),淀粉酶100 U/g,糖化酶3 750 U/g,活性干酵母1.50%;在30 ℃静止发酵120 h,发酵液中的乙醇质量浓度达到106.5 g/L,橡子淀粉的乙醇转化率达到89.36 %。采用橡子粉发酵法制备燃料乙醇与以玉米等粮食作物为原料制备的燃料乙醇质量浓度相当,可以替代粮食作物生产燃料乙醇。

Fermentation Optimization of Fuel Ethanol Producing by Simultaneous Liquefaction,Saccharification and Fermentation from Acorn Flour

The fuel ethanol was produced from acron flour by simultaneous liquefaction,saccharification and fermentation (SLSF).The optimum conditions of fermentation by SLSF technology were determined through single factor experiments and orthogonal experiments.The results showed that SLSF technology was suitable for the ethanol fermentation from acorn flour.The optimum conditions were 20 g acorn flour removed of tannin,the ratio of materials to water 1:3,amylase 100 U/g,glucoamylase 3 750 U/g and active dry yeast 1.50%.The ethanol concentration of fermentation broth reached 106.5 g/L after static fermentation for 120 h at 30 ℃,and the conversion rate of acorn starch into ethanol reached 89.36%.The ethanol concentration of fermentation liquor by simultaneous liquefaction,saccharification and fermentation was comparable to the ethanol concentration fermented by corn and other food crops.