| 一种新的检测啤酒有害菌培养基的优化 |
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| 引用本文： | 袁雪萍,王德良,宋绪磊. 一种新的检测啤酒有害菌培养基的优化[J]. 酿酒科技, 2011, 0(12): 48-50,53 |
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| 作者姓名： | 袁雪萍 王德良 宋绪磊 |
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| 作者单位： | 1. 新疆农业大学,新疆乌鲁木齐,830052
2. 中国食品发酵工业研究院,北京,100027 |
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| 摘 要： | 为研制出更适合国产啤酒有害菌检测的培养基,参考目前国内啤酒行业使用量最大的2种进口培养基MRS和NBB,利用2水平正交试验考察牛肉浸粉、酵母浸出物、葡萄糖、吐温-80、麦根浸出物、精氨酸、叶酸等因素对菌落数的影响。主要的影响因素是:牛肉浸粉、酵母浸出物、葡萄糖和叶酸,最优的培养基配方为:牛肉浸粉8 g/L、酵母浸出物6 g/L、葡萄糖18 g/L、叶酸0.22 g/L。并且通过反复实验,确定此配方为最佳的培养基配方。
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| 关 键 词： | 啤酒有害菌 培养基 检测 MRS NBB |
Optimization of a New Culture Medium for the Detection of Harmful Bacteria in Beer |
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| YUAN Xueping,WANG Deliang,and SONG Xulei. Optimization of a New Culture Medium for the Detection of Harmful Bacteria in Beer[J]. Liquor-making Science & Technology, 2011, 0(12): 48-50,53 |
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| Authors: | YUAN Xueping WANG Deliang and SONG Xulei |
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| Affiliation: | 1.Xinjiang Agricultural University,Urumqi,Xinjiang 830052;2.China National Research Institute of Food Science and Fermentation Industry,Beijing 100027,China) |
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| Abstract: | In order to develop culture mediums more suitable for the detection of harmful bacteria in, home-made beer, we had referred to two kinds of imported culture mediums including MRS and NBB （most widely used in China nowadays） and carried out two-level orthogonal test to investigate the effects of beef powder, yeast extract, glucose, Tween80, malt rootlet extract, L-arginine and folic acid on bacteria colony number. And we believed that the main influencing factors included beef powder, yeast extract, glucose and folic acid. As a result, the best formula of the culture medium was determined as follows： 8 g/L beef powder, 6 g/L yeast extract, 18g/L glucose, and 0.22 g/L folic acid. Such formula was proved to be the best one through repeated verification experiments. |
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| Keywords: | harmful bacteria in beer culture mediums detection MRS NBB |
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