三种离子物质对壳聚糖/果胶聚电解质复合物牛血清蛋白释放特性的影响

分别添加氯化钠、硫酸钠、氯化铁制备壳聚糖/果胶聚电解质复合物(PEC),研究其对牛血清蛋白(BSA)的负载能力及模拟释放行为,以探讨三种离子物质对壳聚糖/果胶PEC在蛋白缓释方面的影响。氯化铁PEC对BSA的包埋率最大,其次为硫酸钠、氯化钠,载药率为氯化钠PEC最大、其次为硫酸钠、氯化铁。在单一模拟环境中,各PEC在模拟胃液中的BSA累积释放率最小,在模拟结肠液中最大;氯化钠会使PEC的BSA释放率降低,而硫酸钠与氯化铁则可增大蛋白释放率;各PEC的蛋白释放行为属于Fick扩散。在连续模拟环境中,模拟胃液中BSA累积释放率较低,在模拟肠道环境中BSA大量释放,含有氯化钠的PEC累积释放率最低;在模拟结肠液中含有果胶酶时,各PEC的BSA累积释放率可达98%以上;各PEC的蛋白释放动力学属于非Fick扩散。含有三种离子物质的壳聚糖/果胶PEC均具有良好的p H敏感性和定点释放特性。

The Effects of Three Ionic Species on the BSA Protein Release Properties of Chitosan/Pectin Polyelectrolyte Complexes

Chitosan/pectin polyelectrolyte complexes (PECs) were prepared by adding NaCl, Na2SO4, and FeCl3, respectively. The bovine serum albumin (BSA) loading efficiency and simulated releasing behavior of PECs were studied and the effects of the three ionic species on the protein release properties of chitosan/pectin PECs were investigated. Results showed that the encapsulation efficiency of FeCl3 PEC was the highest, followed by Na2SO4 and NaCl; the loading efficiency of NaCl PEC was the highest, followed by Na2SO4 and FeCl3. In single simulation, the BSA accumulative release ratio in the GI tract juices of PECs was the lowest; however, it was the highest in simulated colon juice, The BSA release ratio of PEC was reduced by the addition of NaCl, while the addition of Na2SO4 and FeCl3 increased the BSA release ratios. The Fick diffusion theory can be used to describe the kinetics of the BSA release behavior of PECs with the three ions. In successive simulations, the BSA accumulative release ratio of PECs in simulated GI tract juices was the lowest, while BSA was released in large quantities in the simulated colon, with NaCl PEC having the lowest accumulative release ratio. The BSA accumulative release ratio of PECs was more than 98% when pectinase was present in simulated colon juice. The release behavior of PECs s was the Non-Fick diffusion type. All the chitosan/pectin PECs formed with the three ionic species showed favorable pH sensitivity and site-specific delivery properties.