高效液相色谱法测定软饮料中香兰素和乙基香兰素

目的建立软饮料中香兰素和乙基香兰素的高效液相色谱法检测方法。方法样品中的香兰素和乙基香兰素在酸性条件下经乙腈提取,利用盐析作用分层净化,提取液浓缩复溶解后,供液相色谱检测。检测波长308 nm,外标法定量。结果香兰素和乙基香兰素在0.5~50.0?g/m L浓度范围内线性关系良好,相关系数均大于0.9999。添加水平为5、30、63 mg/kg时,香兰素和乙基香兰素的平均回收率分别为98.4%和100.4%,96.7%和100.2%,97.8%和100.3%,相对标准偏差在3.53%~4.70%,检出限均为0.5 mg/kg。结论方法操作简便、快速,经济实用,适用性强,结果准确可靠,可用于样品批量快速检测。

Determination of vanillin and ethyl vanillin in soft drink by high performance liquid chromatography

Objective To develop a method for determination of vanillin and ethyl vanillin in soft drink by high performance liquid chromatography (HPLC). Methods Vanillin and ethyl vanillin in sample were extracted with acetonitrile and 0.1% formic acid solution, and were cleaned up by salting out effect. After concentrated, the extraction was redissolved and determined by HPLC at 308 nm. The external standard method was used for quantitative analysis. Results The method showed a good linear over the range of 0.5~50.0 ?g/mL for vanillin and ethyl vanillin with correlation coefficients above 0.9999. The average recoveries were 98.4% and 100.4%, 96.7% and 100.2%, 97.8% and 100.3% at the spiked level of 5, 30, and 63 mg/kg, with relative standard deviation between 3.53%~4.70%. The limit of detection was 0.5 mg/kg. Conclusion The method is simple, rapid, economical and practical, and the result is accurate and reliable, which is suitable for batch sample determination.