葡萄籽原花青素对顺铂所致大鼠肾脏氧化损伤和线粒体损伤的防护作用

本文研究了葡萄籽原花青素(GSPE)对顺铂(CDDP)所致大鼠肾脏氧化应激损伤和线粒体损伤的防护作用。实验大鼠分为正常对照组、CDDP模型组、GSPE(400 mg/kg)组、CDDP+GSPE(200 mg/kg)组和CDDP+GSPE(400 mg/kg)组。各组分别以蒸馏水和相应剂量GSPE对大鼠连续灌胃15 d。灌胃10 d后CDDP组和CDDP+GSPE组一次性腹腔注射CDDP(8.0 mg/kg),其余注射等量生理盐水。检测血清尿素氮(BUN)和肌酐(Cr E)、肾脏指数、肾皮质抗氧化和碳水化合物代谢酶指标,光镜观察肾组织结构。GSPE预处理的大鼠在注射CDDP后与模型组相比肾脏指数、BUN、Cr E含量显著降低,肾皮质还原型谷胱甘肽(GSH)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和苹果酸脱氢酶(MDH)、己糖激酶(HK)活力的变化明显改善。病理切片显示,GSPE预处理可减轻顺铂引起的肾小球萎缩等病理损伤。提示GSPE对CDDP所致大鼠氧化应激损伤和线粒体损伤具有明显的防护作用。

Protective Effects of Grape Seed Proanthocyanidin Extract (GSPE) on cis-Diamminedichloroplatinum (CDDP)-Induced Renal Oxidative Damage and Mitochondrial Injury in Rats

In this paper, the protective effects of grape seed proanthocyanidin extract (GSPE) on cis-diamminedichloroplatinum (CDDP)-induced renal oxidative stress and mitochondrial injury in rats were investigated. Rats were randomly divided into five groups: normal control group, CDDP model group, GSPE (400 mg/kg) group, CDDP + GSPE (200 mg/kg) group, and CDDP + GSPE (400 mg/kg) group. Each group was administrated with distilled water or the corresponding doses of GSPE by gavage for 15 consecutive days. After 10 d of administration, the CDDP and CDDP + GSPE groups were administered a single intraperitoneal injection of CDDP (8 mg/kg), and the remaining groups were administered with normal saline by intraperitoneal injection. The blood urea nitrogen (BUN), creatinine (CrE), renal index, antioxidant index for renal cortex, and index for the carbohydrate metabolic enzyme were measured, and the renal structures were observed using a light microscope. Compared to the CDDP model group, the GSPE pretreated rats showed a significant decrease in renal index as well as BUN and CrE contents. The CDDP treatment improved the contents of renal cortical reduced glutathione (GSH), and malondialdehyde (MDA), meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), malate dehydrogenase (MDH), and hexokinase (HK) were enhanced. The pathologic slice revealed that GSPE pretreatment could reduce CDDP-induced pathological damage such as glomerular atrophy. This study suggested that GSPE had a protective effect on the CDDP-induced renal oxidative stress and mitochondrial injury in rats.