一株Bifidobacterium longum CCFM760的生理特性、安全性评价和基因分析

长双歧杆菌(Bifidobacterium longum CCFM760)是一株从江苏省无锡市滨湖区两岁健康幼女粪便分离筛选得到的具有良好缓解便秘功效的益生菌。作者对其生长产酸能力、人工模拟肠胃液耐受性、抑菌能力、低聚糖利用能力、抗生素耐受能力和总超氧化物歧化酶(SOD)活性进行了检测。该菌具有良好的生长产酸能力和人工模拟肠胃液耐受性,对大肠杆菌和金黄色葡萄球菌有明显的抑制作用,能显著利用7种低聚糖,对14种抗生素敏感,培养液中总SOD活性达到(99.14±9.91)U/mL。此外,对该菌进行基因组草图测序,并从基因组角度解析了其低聚糖代谢能力、抗生素敏感性以及抗氧化能力。以上结果表明,B.longum CCFM760是一株安全的、具有实际应用潜力的益生菌。

Physiological Characteristics,Safety Evaluation and Genetic Analysis of Bifidobacterium longum CCFM760

Bifidobacterium longum CCFM760 is screened from stools of a healthy two-year-old youngster in Binhu District,Wuxi City,Jiangsu Province,China.In this study,the ability to grow and produce acid,artificial simulated gastrointestinal fluid tolerance,antibacterial ability,oligosaccharide utilization ability and antibiotic tolerance of B.longum CCFM760 were assessed,as well as its total superoxide dismutase(SOD)activity.It was found that this bacterium had good growth acidogenicity and artificial simulated gastrointestinal fluid tolerance,and obviously inhibited Escherichia coli and Staphylococcus aureus.Meanwhile,it could significantly utilize seven oligosaccharides and was sensitive to 14 antibiotics.The total activity of SOD in the culture reached(99.14±9.91)U/mL.In addition,the genome sequence of the strain was sequenced to explain the ability of oligosaccharide metabolism,antibiotic sensitivity and anti-oxidation of the bacteria.These results demonstrated that B.longum CCFM760 is a safe and potential probiotic.