| 贯筋藤鲜茎中凝乳酶的提取工艺研究 |
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| 引用本文： | 王红燕,陶 亮,陈 森,李 涛,黄艾祥.贯筋藤鲜茎中凝乳酶的提取工艺研究[J].中国酿造,2015,34(3):34. |
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| 作者姓名： | 王红燕 陶 亮 陈 森 李 涛 黄艾祥 |
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| 作者单位： | 1.云南农业大学 食品科学技术学院云南 昆明 650201；2.云南农业大学 植物保护学院 云南 昆明 650201 |
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| 基金项目： | 国家自然科学基金资助项目（31160331）；云南省高校食品加工与安全控制重点实验室项目（云教科（2014）16号） |
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| 摘 要： | 以云南西北部地区的野生植物贯筋藤的鲜茎为原料，在单因素的试验基础上，采用L9（33）正交试验方法，以贯筋藤鲜茎中凝乳酶活力为评价指标，研究了不同的预处理方式、料液比、浸泡时间、浸泡温度对其提取工艺的影响，确定了贯筋藤鲜茎中凝乳酶的最佳提取条件和提取率。结果表明，贯筋藤鲜茎中凝乳酶的提取最佳条件为料液比1∶20（g∶mL），提取时间40 min，提取温度65 ℃，在此条件下得到的凝乳酶活力为260.984 SU/mL，提取率为31.35%。
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| 关 键 词： | 贯筋藤 凝乳酶 提取工艺 酶活力 提取率 |
Extraction process of rennet from fresh stalk of Dregea sinensis Hemsl |
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| WANG Hongyan,TAO Liang,CHEN Sen,LI Tao,HUANG Aixiang.Extraction process of rennet from fresh stalk of Dregea sinensis Hemsl[J].China Brewing,2015,34(3):34. |
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| Authors: | WANG Hongyan TAO Liang CHEN Sen LI Tao HUANG Aixiang |
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| Affiliation: | 1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2.College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | The fresh stalk of wild Dregea Sinensis Hemsl from the northwest of Yunnan province was researched. On the basis of single factor test, using rennet activity as evaluation index, the effects of different pretreatments, solid-liquid ratio, extraction time and temperature on enzyme activity of D. sinensis Hemsl fresh stalk was studied. Optimal extraction conditions were determined by three factors and three levels orthogonal experiment. The results showed that the optimal extraction process of rennet from D. sinensis Hemsl was solid-liquid ratio 1∶20 (g∶ml), extraction time 40 min, extraction temperature 65 ℃. Under this condition, the rennet activity was 260.984 SU/ml, the rennet extraction rate was 31.35%. |
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| Keywords: | Dregea sinensis Hemsl rennet extraction process enzyme activity extraction rate |
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