HPLC测定不同厂家发酵型茶叶中阿魏酸含量

采用高效液相色谱法测定不同厂家发酵型茶叶中阿魏酸的含量，色谱条件为Shimpack VP-ODS C18（150 mm×6 mm，5 μm）色谱柱，流动相为0.2%冰醋酸-甲醇（28∶72），检测波长314 nm，柱温30 ℃，流速1.0 mL/min。结果表明，阿魏酸在20～100 μg/mL范围内线性良好（相关系数R2=0.999 8），平均加样回收率为98.73%，相对标准偏差（RSD）为1.71%。测得9个厂家不同发酵型茶叶中阿魏酸的含量在22.559～30.286 μg/g，其中湖南益阳的湘益茯砖茶阿魏酸含量最高，为30.286 μg/g。该方法简便快速，适用于发酵型茶叶中阿魏酸含量的测定。

Ferulic acid content determination of fermented tea from different manufacturers by HPLC

The ferulic acid contents of fermented tea from different manufacturers were determined by HPLC. The separation was performed on Shimpack VP-ODS C18 column (150 mm×6 mm, 5 μm) with 0.2% glacial acetic acid-methyl alcohol (28∶72) as mobile phase. Ferulic acid was detected at 314 nm with flowing rate 1.0 ml/min at 30 ℃. The calibration curve was linear (R2=0.999 8) in the range of 20-100 μg/ml for ferulic acid. The average recovery was 98.73% and relative standard deviation was 1.71%, respectively. The ferulic acid contents of fermented tea from 9 manufacturers were 22.559-30.286 μg/g, and the ferulic acid content in Xiangyi brick tea was the highest of 30.286 μg/g. The method was quick and convenient for ferulic acid determination for fermented tea.